色谱柱的作用是什么

---

今日新闻                               日期：2022/6/24

色谱柱的作用是什么

色谱柱可分为填充柱和开管柱两大类。多为金属或玻璃制作。有直管形、盘管形、U形管等形状。液相色谱通常均采用填充柱。

色谱柱的分离效果取决于所选择的固定相，以及色谱柱的制备和操作条件。色谱是一种分离分析手段，分离是核心，因此担负分离作用的色谱柱是色谱系统的心脏。

色谱柱由柱管、压帽、卡套（密封环）、筛板（滤片）、接头、螺丝等组成。柱管多用不锈钢制成，压力不高于70 kg/cm2 时，也可采用厚壁玻璃或石英管，管nei壁要求有很高的光洁度。

为提G柱效，减小管壁效应，不锈钢柱nei壁多经过抛光。也有人在不锈钢柱nei壁涂敷氟塑料以提高nei壁的光洁度，其效果与抛光相同。

还有使用熔融硅或玻璃衬里的，用于细管柱。色谱柱liang端的柱接头nei装有筛板，是烧结不锈钢或钛合金，孔径0.2~20μm(5~10&μm)，取决于填料粒度，目的是防止填料漏出。

 

扩展资料

---

色谱柱按用途可分为分析型和制备型两类，尺寸规格也不同：

1、常规分析柱（常量柱），内径2~5mm（常用4.6mm，国内有4mm和5mm），柱长10~30cm；

2、窄径柱（narrow bore，又称细管径柱、半微柱semi-microcolumn），内径1~2mm，柱长10~20cm；

3、毛细管柱（又称微柱microcolumn），内径0.2~0.5mm；

4、半制备柱，内径>5mm；

5、实验室制备柱，内径20~40mm，柱长10~30cm；

6、生产制备柱内径可达几十厘米。柱内径一般是根据柱长、填料粒径和折合流速来确定，目的是为了避免管壁效应。

英文翻译：

---

What is the role of the column 

Chromatographic columns can be divided into two categories:packed column and open column.Most are made of metal or glass.There are straight tube, coiltube, U tube and other shapes.Liquid chromatography usually uses packed columns.

The separation iffect of the column depends on the stationary phase selected,the preparation and operating conditions of the column.Cheromatography is a means of separation and analysis,separation is the core,so the separation column is the heart of the chromatography system.

Chromatographic column is composed of column tube,pressure cap, clamp (seal ring),sieve plate (filter),joint,screw and so on.The column tube is made of stainless steel.When the pressure is not higher than 70kg/cm²,the thick wall glass or quartz tube can also be used.The nel wall of the tube requires high finish.

In order to improve the g-column efficiency and reduce the tube wall effect,the nel wall of stainless steel column is mostly polished. Ther are also people in the stainless steel column NEI wall coated with air plast to improve the finish of the NEI wall,the effect is the same as polishing.

Others are lined with molten silicon or glass for thin pipe strings.The column joint NEI at the liang end of the column is fitted with a sieve plate, which is sintered stqainless steel of titanium alloy.The aperture is 0.2-20μm(5~10&μm),depending on the size of the filler,to prevent leakage of the filler.